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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Aug 9;503(4):2619–2624. doi: 10.1016/j.bbrc.2018.08.013

Figure 4. RILP cleavage and phosphorylation promote kinesin association and anterograde movement.

Figure 4.

(A) RILP constructs were expressed in HeLa cells and infected with SeV for 24 h. Cells were lysed in hypotonic lysis buffer and RILP was immunoprecipitated using FLAG M2 magnetic beads as described previously. Eluates were assessed by western blot analysis for kinesin heavy chain. (B) The localization of the MVB marker CD63 was assessed in HeLa cells expressing either cRILP-FLAG or the cleavage mutant RILP(D75A)-FLAG.