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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Stem Cell Res. 2018 Jul 19;31:83–94. doi: 10.1016/j.scr.2018.07.009

Fig. 4.

Fig. 4.

Characterization of biological impact of downstream targets of Dppa4 in 3T3: Cdkn2c and Etv4. A) Expression of Cdkn2c in WT and Dppa4 expressing 3T3 after transduction and selection for ectopic Cdkn2c expression, compared to WT empty vector (EV) control n = 4. B) Expression of Etv4 in WT and Dppa4 expressing 3T3 after transduction and selection for ectopic Etv4 expression, compared to WT empty vector control n = 4. C) Expression of Etv4 in WT and Dppa4 expressing 3T3 after siRNA knockdown, compared to negative siRNA control in WT 3T3 n = 3. D) Expression of Cdkn2c in WT 3T3 overexpressing Etv4 compared to empty vector control, n = 4. E) Count of number of cells 20 h and 48 h after seeding, n = 3. ANOVA with Post-hoc Tukey HSD test. F) Propidium iodide cell cycle analysis, n = 6. ANOVA with Post-hoc Tukey HSD test. G) Propidium iodide cell cycle analysis, n = 6. ANOVA with Post-hoc Tukey HSD test. *p < .05, **p < .01. Error bars are the S.E.M.