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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Stem Cell Res. 2018 Jul 19;31:83–94. doi: 10.1016/j.scr.2018.07.009

Fig. 5.

Fig. 5.

Dppa4 also regulates cell cycle and transcription factor targets in E14 mESCs. A) Overlap of genes downregulated by Dppa4 disruption in E14 mESC and Dppa4 ChIP-Seq peaks in WT E14 cells. Hypergeometric distribution was used to calculate probabilities. B) Overlap of genes upregulated by Dppa4 knockout in E14 mESC and Dppa4 ChIP-Seq peaks in WT E14 cells. Hypergeometric distribution was used to calculate probabilities. C) Significant gene ontology categories for Dppa4-activated direct targets. D) Significant gene ontology categories for Dppa4-repressed direct targets. E) Dppa4 ChIP-qPCR validation of select Dppa4 targets, n = 2 (independently-generated knockout lines). F) qPCR validation of gene expression changes in select Dppa4 targets, n = 2 (independently-generated knockout lines). t-test. *p < .05, **p < .01. Error bars are the S.E.M.