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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: Methods. 2018 May 10;148:57–66. doi: 10.1016/j.ymeth.2018.05.004

Figure 8.

Figure 8

Kinetic investigation of protein translocation on DNA through analysis of self-decoupling of intermolecular hydrogen-bond scalar coupling between protein 15N and DNA 31P nuclei [94]. (A) Theoretical relationship between the apparent value of intermolecular hydrogen-bond scalar coupling and the residence time of a molecular complex. Four cases simulated with different values of the intrinsic coupling constants are shown. (B) h3JNP coupling constants measured for the hydrogen bonds between Lys side-chain 15N and DNA phosphate 31P nuclei in the Antp homeodomain-DNA complex at some different salt concentrations. The h3JNP coupling constants were measured as described by Anderson et al. [31] (C) Comparison of the residence times of the Antp homeodomain at the recognition sequence measured with the h3JNP method and those measured with 15N z-exchange method.