Fig. 1.

A photo taken during MACS sorting of non-apoptotic spermatozoa. As it can be seen on the photo, MACS ART MS Column is placed into MiniMACS Separator, which is attached to MACS MultiStand. After washing of the column with MACS ART Binding Buffer (left bigger bottle standing on the MultiStand), the sample of spermatozoa mixed with MACS ART Annexin V Reagent (right smaller bottle standing on the MultiStand) is applied into the column and unlabeled (non-apoptotic) spermatozoa that pass through the column are collected in a centrifuge tube standing under the column. The column is washed once with MACS ART Binding Buffer to collect all unlabeled spermatozoa. The unlabeled spermatozoa are then washed with sperm preparation medium and prepared for ICSI