Table 2.
Tannins (mg/g seed) | Phytic acid (mg/g seed) | Saponins (mg/g seed) | Hemagglutination (µg seed/mL) | Trypsin inhibitor activity (µg/g seed) | |
---|---|---|---|---|---|
Control | 0.19 ± 0.0 | 59.4 ± 1.0a | 7.02 ± 0.2a | 0.68 | 32.2 ± 1.1a |
T80 | nd | 45.7 ± 1.0b | 7.83 ± 0.5b | 0.97 | 23.8 ± 0.7b |
T120 | nd | 39.1 ± 0.6c | 8.49 ± 0.3c | 3.36 | 12.6 ± 1.2c |
T160 | nd | 5.43 ± 0.4d | 10.85 ± 0.4d | 62.5 | 1.93 ± 0.9d |
Results are mean ± SD. Letters (a, b, c, d) represent significant difference between samples. Determination of tannins, phytic acid and saponins was carried out in triplicates. Hemagglutination and trypsin inhibitor activity assays were performed in duplicates. nd not detected