Fig. 2.

Agarose gel electrophoresis analysis of NPFMAP double-stranded DNA, pGEX-6P-1 vector, and pGEX-6P-1-NPFMAP recombinant plasmid. a Lane 1: NPFMAP PCR product after two rounds of overlap extension PCR; lane 2: DNA ladder of 10,000 bp. b Lanes 1–2: single digestion of the pGEX-6P-1 vector by BamH I (1) and Xho I (2); lane 3: DNA ladder of 10,000 bp. c Lane 1: double digestion of the pGEX-6P-1-NPFMAP recombinant plasmid by BamH I and Xho I; lane 2: DNA ladder of 10,000 bp. Arrows indicate the PCR (a) and digestion (b, c) products