Fig. 3.

Expression of the GST-NPFMAP recombinant fusion protein. a 12% SDS-PAGE analysis showing expression of GST-NPFMAP at 20 °C and 37 °C. Lane 1: non-induced lysates; lane 2: lysates induced for 16 h at 20 °C; lane 3: supernatant induced for 16 h at 20 °C; lane 4: lysates induced for 4 h at 37 °C; lane 5: supernatant induced for 4 h at 37 °C; lane M: protein molecular weight marker. b Western blot analysis of GST-NPFMAP induced at 37 °C and probed using an anti-GST tag antibody. Lane M: protein molecular weight marker; lane 1: non-induced supernatant and lysates; lane 2: induced lysates; lane 3: induced supernatant. Arrows denote the position of the fusion proteins