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. 2018 Aug 24;55(10):4266–4275. doi: 10.1007/s13197-018-3365-x

Fig. 5.

Fig. 5

SDS-PAGE analysis comparing expression of GST-NPFMAP and NPFMAP at various stages of purification, a SDS-PAGE analysis of eluates from three purification steps lane M: protein molecular weight marker; lane 1: lysates of GST-NPFMAP induced at 37 °C; lane 2: effluent obtained while the sample was in the column; Lane 3: eluate resulting from washing out the column with binding buffer; lanes 4–7: eluate containing GST-NPFMAP. b Lane M: protein molecular weight marker; lane 1: GST-NPFMAP not digested with PreScission protease; lane 2: GST-NPFMAP digested with PreScission protease; lane 3: effluent obtained while the sample was in the column; lane 4: eluate obtained after washing out the column with elution buffer; c lane M: protein molecular weight marker; lane 1: NPFMAP following a second affinity chromatography purification step. Arrows denote the position of the fusion (a) and target (b, c) proteins