Table 1. Primers used for genetic analysis and band sizes obtained.

Polymorphism	Primers	Method of genotyping	Band sizes
VEGF -460T/C	P1F-5′-TGT GCA GAC GGC AGT CAC TA-3′	Direct sequencing	Not required for genotyping
	P1R-5′-CCC GCT ACC AGC CGA CTT T-3′
VEGF -634G/C	P2F-5′-TTG CTT GCC ATT CCC CAC TTG A-3′	PCR-RFLP with BsmFI	G allele-196 and 274 bp
	P2R-5′-CCG AAG CGA GAA CAG CCC AGA A-3′		C allele-470 bp
VEGF +405G/C	P3F-5′-ATT TAT TTT TGC TTG CCA TT-3′	PCR-RFLP with BsmFI	G allele-193 and 111 bp
	P3R-5′-GTC TGT CTG TCT GTC CGT CA-3′		C allele-304 bp
VEGF +936C/T	P4F-5′-AAG GAA GAG GAG ACT CTG CGC-3′	PCR-RFLP with NlaIII	C allele-198 bp
	P4R-5′-TAT GTG GGT GGG TGT GTC TAC AGG-3′		T allele-114 and 84 bp
ACE ID	P5F-5′-CTG GAG ACC ACT CCC ATC CTT TCT-3′	PCR and confirmed with a second ARMS-PCR	I allele-490 bp
	P5R-5′-GAT GTG GCC ATC ACA TTC GTC AGA T-3′		D allele-190 bp
	P6F-5′-TGG GAC CAC AGC GCC CGC CCG CCA CTA C-3′		I allele-335 bp
	P6R-5′-TCG CCA GCC CTC CCA TGC CCA TAA-3′		D allele-no band
TNF -308G/A	P7F-5′-AGG CAA TAG GTT TTG AGG GCC AT-3′	PCR-RFLP with NcoI	G allele-87 and 20 bp
	P7R-5′-TCC TCC CTG CTC CGA TTC CG-3′		A allele-107 bp
GSTT1 null/present	P8F-5′-TTC CTT ACT GGT CCT CAC ATC TC-3′	PCR (multiplex with albumin gene)	Null-no band
	P8R-5′-TCA CCG GAT CAT GGC CAG CA-3′		Present-459 bp
	P9F-5′-GCC CTC TGC TAA CAA GTC CTA C-3′		Internal control-350 bp
	P9R-5′-GCC CTA AAA AGA AAA TCC CCA ATC-3′
GSTM1 null/present	P10F-5′-GAA CTC CCT GAA AAG CTA AAG C-3′	PCR (multiplex with albumin gene)	Null-no band
	P10R-5′-GTT GGG CTC AAA TAT ACG GTG G-3′		Present-219 bp
	P9F-5′-GCC CTC TGC TAA CAA GTC CTA C-3′		Internal control-350 bp
	P9R-5′-GCC CTA AAA AGA AAA TCC CCA ATC-3′
GSTP1 Ile/Val	P11F-5′-ACC CCA GGG CTC TAT GGG AA-3′	PCR-RFLP with Alw26I	Ile allele-176 bp
	P11R-5′-TGA GGG CAC AAG AAG CCC CT-3′		Val allele-85 and 91 bp