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. 2018 Jul 17;4:76. doi: 10.1038/s41420-018-0078-x

Fig. 8. Effect of cocaine on survivin, Nrf-2, and antioxidants.

Fig. 8

For survivin gene expression, the mRNA levels in 4 mM cocaine-treated and control cells were quantified (n = 4) by real-time PCR using GAPDH as the reference gene (a); in another study, the cells were pretreated with 1 µM YM155 (survivin gene inhibitor) for 30 min, followed by cocaine co-treatment for 1 h, and the cell viability was measured (n = 4) in a micro plate reader (b). For Nrf-2 gene expression, the mRNA level was quantified (n = 3) by real-time PCR using GAPDH as the reference gene (c). Colorimetric assays were performed for glutathione (n = 9) (d) or catalase (e, n = 3) or GPx (f, n = 3) or role of PIK-75 (Nrf-2 inhibitor) on cocaine treated cells for viability (g, n = 8) or glutathione (h, n = 8). Data were represented as mean ± SEM, significant compared to the control (*P < 0.05 or **P < 0.01, one-way ANOVA, Dunnett’s (a, c, d–f) or Bonferroni’s (b, g, h) multiple comparison tests)