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. 2018 Sep 11;8:13642. doi: 10.1038/s41598-018-32067-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of intracellular Zn²⁺. (a) Bright field and fluorescence images of intracellular Zn²⁺ in undifferentiated (1 day of culture) C2C12 after Fluozin3-AM labelling. (b) Bright field and fluorescence images of intracellular Zn²⁺ in differentiated C2C12 (6 day of culture, myotubes) after Fluozin3-AM labelling. Values are represented as relative fluorescent units (R.F.U.) for intracellular zinc. (c) Live/Dead images of myotubes (differentiated cells) and quantification represented as viable cells ratio (number of viable cells +Zn/number of viable cells w/o Zn). (Scale bar: 200 μM). (N = 7 independent experiments performed). Graphs show mean ± standard deviation. Significant differences were determined by ANOVA test; *p < 0.05.