Figure 6.

Effects of Zip7 silencing in intracellular Zn²⁺ homeostasis and proliferation. (a) Intracellular Zn²⁺ measurements after Fluozin3-AM labelling in control (UC, untreated cells) and Zip7-deficient cells. Fluorescence emission was measured every 40 seconds. Values are represented as relative fluorescent units (R.F.U.). (N = 6 independent experiments performed). (b) Myoblasts proliferation was determined by BrdU labelling followed by quantification of immunofluorescence. Values are represented as the ratio between BrdU positive cells/total nuclei (N = 5 independent experiments performed). UC: untreated cells. Graphs show mean ± standard deviation. Significant differences were determined by ANOVA test; *p < 0.05.