Fig. 1.

IAV-induced changes in the host cell phosphoproteome. a Overview of the experimental design. b, c Distribution of changes in cellular phosphorylation after 5 (b) and 15 (c) min of infection of A549 cells with IAV strain A/WSN/33 at MOI = 25 PFU/cell. Median fold-changes in phosphorylation of the identified phosphosites are presented in relation to the intensity of detection. Dephosphorylated peptides are shown in red and hyper-phosphorylated ones in blue. Darker colors are used for the results of the Ti⁺⁴ chromatography, lighter colors for TiO₂. The size of the circles reflects the number of biological replicates in which a given peptide was identified. The names of the proteins for the main differentially phosphorylated peptides are indicated. d The main IAV-responsive pathways are represented by circles in the context of the KEGG pathway topology. The size of the circles reflects the number of differentially phosphorylated peptides, while the color indicates the significance of enrichment from the standard hypergeometric test. Edge width corresponds to the number of shared components between a pair of KEGG networks. e The differentially phosphorylated proteins in the top enriched pathways are represented in the context of a functional association network. Asterisks are used to identify small or distant nodes for the represented pathways. Vertices are colored based on the adjusted p-values for enrichment from the standard hypergeometric test