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. 2018 Sep 11;9(9):916. doi: 10.1038/s41419-018-0976-0

Fig. 2. ARHGAP9 overexpression inhibited the HCC cell proliferation, migration and invasion as well as in vivo lung metastasis.

Fig. 2

a HepG2 and MHCC-97H cells were transduced with ARHGAP9 overexpressing (ARHGAP9OE) or control (vector) lentivirus. Cells without any treatment (WT) served as negative control. Ectopic expression of ARHGAP9 was evaluated using western blotting at 48 h after viral transduction. Representative blots from three independent experiments are shown. b Cell counting kit-8 (CCK-8) assays were conducted to determine cell proliferation at 0, 24, 48 and 72 h after treatment. c, d Transwell assays were conducted to determine the migration (c) and invasion (d) ability of HepG2 and MHCC-97H cells. **P < 0.01, ***P < 0.001 versus WT and vector. e, f HepG2 cells overexpressing ARHGAP9 (ARHGAP9OE) or vector (e) were injected intravenously into nude mice (n = 6 per group). The number of lung metastases was counted under a dissecting microscope and presented as mean ± SD (f). Lung metastases were validated by H&E staining. Scale bar: 100 μm. ***P < 0.001