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. 2018 Sep 11;9(9):916. doi: 10.1038/s41419-018-0976-0

Fig. 5. FOXJ2 was critical for the inhibitory effects of ARHGAP9 on HCC cell migration and invasion.

Fig. 5

HepG2 and MHCC-97H cells were divided into four groups: WT, cells without any treatment; vector + siNC, cells transduced with control (vector) lentivirus and transfected with control siRNA (siNC); ARHGAP9OE + siNC, cells transduced with ARHGAP9 overexpressing (ARHGAP9OE) lentivirus and transfected with control siRNA (siNC); and ARHGAP9OE + siFOXJ2, cells transduced with ARHGAP9 overexpressing (ARHGAP9OE) lentivirus and transfected with FOXJ2 siRNA (siFOXJ2). a Protein expression of ARHGAP9 and FOXJ2 was evaluated using western blotting at 48 h after viral transduction. Representative blots from three independent experiments are shown. b, c Transwell assays were conducted to determine the migration (b) and invasion (c) ability of HepG2 and MHCC-97H cells. Scale bar: 100 μm. ***P < 0.001 versus Vector + siNC; ##P < 0.01, ###P < 0.001 versus ARHGAP9OE + siNC