Skip to main content
. 2018 Sep 5;12:284. doi: 10.3389/fncel.2018.00284

Figure 4.

Figure 4

Decrease in glycosylated pattern of α-dystroglycan (α-DG) mediated by fukutin (Fktn) is detected in the mPFC of GAD67+/GFP offspring with PS. (A) Coronal sections of mPFC in control (HT-CTRL, up) prenatally stressed (HT-PS, bottom) GAD67+/GFP offspring at P21 immunostained with glycosylated α-dystroglycan (IIH6C4, red) and DAPI (blue). Glycosylation of α-DG was decreased in the mPFC of the PS group. (B) Quantification of the area of clusters positive for the glycosylated pattern of α-DG. Data are reported as the means ± SEM acquired from multiple slices from at least three mice per group (HT-CTRL, 16 slices; HT-PS, 15 slices; WT-CTRL, 16 slices; WT-PS, 18 slices from six mice in each group). The density of glycosylated α-DG in the mPFC of HT-PS mice was significantly lower than in the other three groups (*P < 0.05, one-way analysis of variance (ANOVA) followed by post hoc Ryan-Einot-Gabriel-Welsch F-test). (C) Individual data of Fktn mRNA expression levels were normalized to GAPDH and to the wild type control in each blot. The mean value (gray horizontal line) of HT-PS was significantly decreased compared with WT-CTRL and WT-PS mice (*P < 0.05, Kruskal-Wallis test; n = 10 mice in each group). Scale bars: 20 μm.