Figure 3.

Increased levels of GluN2B-containing and Y1472-GluN2B phosphorylated NMDARs in synaptic triton insoluble fraction (TIF) from Eps8 KO tissue. (A) Western blotting (WB) analysis confirmed that TIF preparation was actually enriched in postsynaptic proteins. (B–D) WB analysis and relative quantification in homogenate and TIF fractions for the indicated antibodies. All the WB were run twice (data are mean ± SEM, N = 4; Kruskal-Wallis test followed by Dunn’s multiple Comparison test *P < 0.05, **P < 0.01).