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. 2018 Sep 11;8:13639. doi: 10.1038/s41598-018-31756-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblot closeups (cropped images; full lenght blot is included as Supplementary Information) and histograms of protein expression levels are shown for neutral (nSMase) and acid Sphingomyelinase (ASM) in CSF from iNPH and AD patients. Both neutral and acid Sphingomyelinase tended to decrease in AD patients compared to iNPH controls. Ceramidase was not appreciably detected. Data were normalized against the total amount of loaded proteins stained with Sypro Ruby and reported as mean ± SD.