Figure 2.

Fra2 TG mice exhibit airway remodeling and hyperresponsiveness. Representative images and subsequent stereological quantification of (A) Periodic acid-Schiff (PAS) staining for mucus volume and mucus producing goblet cells in the airways; (B) Sirius Red staining for peribronchial collagen and (C) double immunohistochemistry for von Willebrand-factor (vWF, brown) and α-smooth muscle actin (SMA, purple) for airway smooth muscle thickness. Insets show representative quantification. Points represent individual animals. (D) Analysis of lung function to determine changes in airway resistance (Rrs), compliance (Crs) and elastance (Ers) in response to increasing doses of methacholine in Fra2 TG (n = 5) and WT (n = 7) mice. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.