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. 2018 Sep 5;9:2018. doi: 10.3389/fimmu.2018.02018

Figure 6.

Figure 6

Blocking of IL-13 signaling ameliorates airway remodeling, hyperresponsiveness and inflammation in Fra2 TG mice. Representative images and subsequent quantification of (A) periodic acid-Schiff (PAS) staining for mucus producing goblet cells, (B) Sirius red staining for peribronchial collagen width and (C) double immunohistochemistry for von Willebrand-factor (vWF, brown) and α-smooth muscle actin (SMA, purple) for airway smooth muscle thickness. Points represent individual animals. *p < 0.05, ***p < 0.001. (D) Lung function testing to determine changes in airway resistance (Rrs), compliance (Crs) and elastance (Ers) in response to increasing doses of methacholine in WT (n = 8) and Fra2 TG mice treated with isotype control (IgG, n = 5) or anti-IL-13 antibodies (n = 6). *p < 0.05 WT vs. TG-IgG, # p < 0.05 WT vs. TG-aIL-13, &p < 0.05 TG-IgG vs. TG-aIL-13. (E) Proliferation of airway smooth muscle cells isolated from WT and Fra2 TG mice as determined by relative thymidine incorporation. Lines indicate the median, *p < 0.05.