Table 2.
Antibody details for flow cytometry.
| Antigen | Label | Company | Clone | Isotype | Dilution factor |
|---|---|---|---|---|---|
| CD3 | FITC | eBioscience | 145-2C11 | Hamster IgG | 1:20 |
| CD4 | APC | Biolegend | GK1.5 | Rat IgG2b, κ | 1:100 |
| CD8 | PE | Biolegend | 53-6.7 | Rat IgG2a, κ | 1:200 |
| CD11b | V500 | BD Bioscience | M1/70 | Rat IgG2b, κ | 1:50 |
| CD11c | ef450 | eBioscience | N418 | Hamster IgG | 1:50 |
| CD19 | AF700 | Biolegend | 6D5 | Rat IgG2a, κ | 1:100 |
| CD24 | PerCP Cy5.5 | BD Bioscience | M1/69 | Rat IgG2b, κ | 1:500 |
| CD25 | APC-Cy7 | Biolegend | PC61 | Rat IgG1, λ | 1:50 |
| CD45 | PerCP-Cy5.5 | eBioscience | 30-F11 | Rat IgG2b, κ | 1:200 |
| CD45 | FITC | Biolegend | 30-F11 | Rat IgG2b, κ | 1:200 |
| CD64 | AF647 | BD Bioscience | X54-5/7.1 | Mouse IgG1, κ | 1:20 |
| gdTCR | BV421 | Biolegend | GL3 | Hamster IgG | 1:50 |
| Gr-1 | PE-Cy7 | Biolegend | RB6-8C5 | Rat IgG2b, κ | 1:800 |
| MHC-II | APC-Cy7 | Biolegend | M5/114.15.2 | Rat IgG2b, κ | 1:400 |
| Siglec F | PE | BD Bioscience | E50-2440 | Rat IgG2a, κ | 1:20 |