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. 2018 Sep 5;9:356. doi: 10.3389/fgene.2018.00356

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Copyright © 2018 Wang, Pu, Zhao, Zhou, Lu, Chen, He, Feng, Wang, Li, Li, Jin, Guo, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression change of candidate genes after the treatment of 5-aza-2′-deoxycytidine. The expression profiles of these four genes before and after 5-Aza treatment in CaES-17 cell line was shown. The RNA quantification was conducted at three replicates for each gene and the GAPDH mRNA levels were used as an internal standard. The 2^-ΔΔCq method was used to analyze the relative changes in these four genes. The Student’s t-test was carried out to test the differential expression after the 5-Aza treatment. ^∗Indicates P < 0.05, ^∗∗indicates P < 0.01 while ^∗∗indicates P < 0.001.