Table 2.

The mean methylation status of the 4 genomic regions in the validation datasets.

Genomic Regiona	No. CpG sitesb	CpGsite Included	Gene	McaMc	McoMc	P-valued	log10(OR)e	95% CIe	Sensf	Specf	AUCf
chr8:67344610-67344805	24	cg20295442, cg20912169	ADHFE1	0.24	0.15	1.70 × 10-3	2.20	1.00–3.72	0.29	0.94	0.64
chr3:27764697-27764940	8	cg22383888	EOMES	0.38	0.24	2.90 × 10-9	3.88	2.51–5.51	0.69	0.77	0.78
chr16:51184268-51184468	18	cg04550052, cg04698114	SALL1	0.37	0.19	3.90 × 10-7	2.41	1.51–3.51	0.53	0.90	0.74
chr7:93519367-93519503	13	cg12973591	TFPI2	0.28	0.13	3.40 × 10-6	3.82	2.26–5.89	0.50	0.91	0.71

^aGenomic region represents the genomic coverage of the reads with targeted bisulfite sequencing, and the genomic coordinates shown here is based on the hg19 version of the genome. ^bNo. CpG sites represents the number of the CpG sites in each region. ^cMcaM represents the mean methylation percentage of the cases in each region, which consisting of several CpG sites, while the McoM represents the mean methylation percentage of the controls in each region. ^dP value is calculated through the Wilcoxon rank-sum test following with FDR (false discovery rate) adjustment for multiple correction. ^eOR and 95% CI were conducted through logistic regression. ^fSens, sensitivity; while Spec, specificity; AUC, area under curve. The sensitivity, specificity as well as the AUC were both with a logistic regression prediction model without adjustment for gender, age and smoking status and alcohol status.