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. 2018 Sep 11;8:13644. doi: 10.1038/s41598-018-28968-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mouse lung resident mesenchymal stem cells (LR-MSCs) display MSC properties. (A) Mouse LR-MSCs morphology after 7 days of culture following isolation by MACS from the mouse lung single-cell suspension was revealed by a standard light microscope. (B) The expression of Sca-1, CD73, CD29, CD44, CD31, CD34 and CD45 on mouse LR-MSCs was measured by flow cytometric analysis. The black line indicates negative control and the red line indicates the respective surface marker staining. (C) Sorted mouse LR-MSCs were seeded in an appropriate differentiation-induction medium. Osteogenic differentiation of mouse LR-MSCs was demonstrated by (d) alkaline phosphatase staining at day 7 and (e) alizarin red staining at day 21; Adipogenesis was detected by the formation of lipid droplets stained with oil red O at day 21. (a–c) Control cells were cultured in normal growth medium.