Fig. 4.

Defects in Mrc1, SWR-C/Htz1, and Rad52 cause synergistic sensitivity to DNA replication stress. a, b Tenfold serial dilutions of wild-type (WT) and mutant strains containing the mrc1Δ, mrc1-aq, or mrc1-1-843 mutation with or without the swr1Δ mutation (a) or htz1Δ mutation (b) were plated on media containing 0–0.03% MMS or 0–100 mM HU to test their sensitivity to replication stress. Plates were imaged after 2 days of growth at 30 °C. c Tenfold serial dilutions of wild-type and mutant strains containing the mrc1Δ, swr1Δ, or mrc1Δ swr1Δ mutations with or without the rad52Δ mutation were plated on media containing 0–100 mM HU to test their sensitivity to replication stress. Plates were imaged after 6 days of growth at 30 °C because of the severe growth defect of rad52Δ-containing strains