Table 2.
Product distributions in the uGCR assay
| GCR type | No. of isolates/total | Rate for GCR typea | Predicted rate based on wild-type product distributionsb | Observed/predicted rate ratio |
|---|---|---|---|---|
| De novo telomere addition | ||||
| Wild type | 15 / 20 | 1.35 [0.52–3.08] x 10−9 | 1.35 [0.52–3.08] x 10−9 | 1.00 |
| swr1Δ | 7 / 18 | 0.32 [0.23–0.43] x 10−9c | 0.62 [0.44–0.83] x 10−9d | 0.52 |
| mrc1Δ | 1 / 18 | 0.17 [0.08–0.21] x 10−9c | 2.25 [1.05–2.85] x 10−9d | 0.07 |
| mrc1Δ swr1Δ | 1 / 21 | 13.3 [6.19–24.3] x 10−9c | 210 [97.5–383] x 10−9d | 0.06 |
| Interstitial deletion | ||||
| Wild type | 1 / 20 | 9.00 [3.50–20.5] x 10−11 | 9.00 [3.50–20.5] x 10−11 | 1.00 |
| swr1Δ | 2 / 18 | 9.11 [6.56–12.2] x 10−11 | 4.10 [2.95–5.50] x 10−11d | 2.22 |
| mrc1Δ | 0 / 18 | <16.7 [7.78–21.1] x 10−11 | 15.0 [7.00–19.0] x 10−11 | 1.11 |
| mrc1Δ swr1Δ | 8 / 21 | 10,700 [4400] x 10−11c | 1400 [650–2550] x 10−11d | 7.62 |
| Microhomology-mediated translocation | ||||
| Wild type | 0 / 20 | <9.00 [3.50–20.5] x 10−11 | <9.00 [3.50–20.5] x 10−11 | 1.00 |
| swr1Δ | 3 / 18 | 13.7 [9.83–18.3] x 10−11 | <4.10 [2.95–5.50] x 10−11d | >3.33 |
| mrc1Δ | 4 / 18 | 66.7 [31.1–84.4] x 10−11c | <15.0 [7.00–19.0] x 10−11d | >4.44 |
| mrc1Δ swr1Δ | 9 / 21 | 12,000 [5900] x 10−11c | <1400 [650–2500] x 10−11d | >8.57 |
| Hairpin-mediated inverted duplication | ||||
| Wild type | 3 / 20 | 2.70 [1.05–6.15] x 10−10 | 2.70 [1.05–6.15] x 10−10 | 1.00 |
| swr1Δ | 6 / 18 | 2.73 [1.97–3.67] x 10−10 | 1.23 [0.89–1.65] x 10−10d | 2.22 |
| mrc1Δ | 13 / 18 | 21.7 [10.1–27.4] x 10−10c | 4.50 [2.10–5.70] x 10−10d | 4.81 |
| mrc1Δ swr1Δ | 3 / 21 | 400 [186–729] x 10−10c | 420 [195–765] x 10−10 | 0.95 |
aObserved rate calculated by multiplying the GCR rate for each strain by the fraction of GCRs observed for a specific GCR type. 95% confidence intervals (CI) are displayed in square brackets.
bPredicted rate calculated by multiplying the GCR rate for each strain by the fraction of GCRs observed in the wild-type strain. 95% confidence intervals are displayed in square brackets.
cRate of a specific type of GCR in the mutant strain has a 95% CI that does not overlap with the 95% CI of the wild-type rate
dPredicted rate based on the wild-type product distribution has a 95% CI that does not overlap with the 95% CI of the observed rate.