Table 3.

Product distributions in the sGCR assay

GCR type	No. of isolates/total	Rate for GCR typea	Predicted rate based on wild-type product distributionsb	Observed/predicted rate ratio
De novo telomere additions
Wild type	5 / 11	2.77 [1.95–8.18] x 10−9	2.77 [1.95–8.18] x 10−9	1.00
swr1Δ	3 / 11	2.29 [1.36–4.09] x 10−9	3.82 [2.27–6.82] x 10−9	0.60
mrc1Δ	2 / 11	10.2 [3.82–18.2] x 10−9	25.5 [9.55–45.5] x 10−9	0.40
mrc1Δ swr1Δ	2 / 10	60.0 [50.0–80.0] x 10−9c	136 [114–182] x 10−9d	0.44
mrc1-1-843	2 / 11	4.36 [3.27–8.55] x 10−9	10.9 [8.18–21.4] x 10−9	0.40
mrc1-1-843 swr1Δ	0 / 11	<29.1 [20.0–40.0] x 10−9	145 [100–200] x 10−9d	<0.20
Interstitial deletion
Wild type	0 / 11	<55.5 [39.1–164] x 10−11	<55.5 [39.1–164] x 10−11	1.00
swr1Δ	0 / 11	<70.0 [41.7–125] x 10−11	<76.4 [45.5–136] x 10−11	0.92
mrc1Δ	1 / 11	509 [191–909] x 10−11	<509 [191–909] x 10−11	>1.00
mrc1Δ swr1Δ	2 / 10	6000 [5000–8000] x 10−11c	<2730 [2270–3640] x 10−11d	>2.20
mrc1-1-843	0 / 11	<200 [150–392] x 10−11	<218 [164–427] x 10−11	0.92
mrc1-1-843 swr1Δ	2 / 11	5820 [4000–8000] x 10−11c	<2910 [2000–4000] x 10−11d	>2.00
Microhomology-mediated translocation
Wild type	0 / 11	<55.5 [39.1–164] x 10−11	<55.5 [39.1–164] x 10−11	1.00
swr1Δ	0 / 11	<64.6 [38.5–115] x 10−11	<76.4 [45.5–136] x 10−11	0.85
mrc1Δ	0 / 11	<467 [175–833] x 10−11	<509 [191–909] x 10−11	0.92
mrc1Δ swr1Δ	0 / 10	<2730 [2270–3640] x 10−11	<2730 [2270–3640] x 10−11	1.00
mrc1-1-843	1 / 11	200 [150–392] x 10−11	<218 [164–427] x 10−11	>0.92
mrc1-1-843 swr1Δ	0 / 11	<2910 [2000–4000] x 10−11	<2910 [2000–4000] x 10−11	1.00
Hairpin-mediated inverted duplication
Wild type	0 / 11	<5.55 [3.91–16.4] x 10−10	<5.55 [3.91–16.4] x 10−10	1.00
swr1Δ	1 / 11	7.64 [4.55–13.6] x 10−10	<7.64 [4.55–13.6] x 10−10	>1.00
mrc1Δ	0 / 11	<50.9 [19.1–90.9] x 10−10	<50.9 [19.1–90.9] x 10−10	1.00
mrc1Δ swr1Δ	1 / 10	300 [250–400] x 10−10c	<273 [227–364] x 10−10	>1.10
mrc1-1-843	0 / 11	<21.8 [16.4–42.7] x 10−10	<21.8 [16.4–42.7] x 10−10	1.00
mrc1-1-843 swr1Δ	6 / 11	1,750 [1400] x 10−10c	<291 [200–400] x 10−10d	>6.00
Homology-mediated inverted duplication
Wild type	0 / 11	<5.55 [3.91–16.4] x 10−10	<5.55 [3.91–16.4] x 10−10	1.00
swr1Δ	0 / 11	<7.64 [4.55–13.6] x 10−10	<7.64 [4.55–13.6] x 10−10	1.00
mrc1Δ	0 / 11	<50.9 [19.1–90.9] x 10−10	<50.9 [19.1–90.9] x 10−10	1.00
mrc1Δ swr1Δ	0 / 10	<300 [250–400] x 10−10	<300 [250–400] x 10−10	1.00
mrc1-1-843	1 / 11	21.8 [16.4–42.7] x 10−10*	<21.8 [16.4–42.7] x 10−10	>1.00
mrc1-1-843 swr1Δ	0 / 11	<291 [200–400] x 10−10	<291 [200–400] x 10−10	1.00
Homology-mediated translocation
Wild type	6 / 11	3.33 [2.35–9.82] x 10−9	3.33 [2.35–9.82] x 10−9	1.00
swr1Δ	3 / 11	2.10 [1.25–3.75] x 10−9	4.58 [2.73–8.18] x 10−9	0.46
mrc1Δ	8 / 11	40.7 [15.3–72.7] x 10−9c	30.5 [11.5–54.5] x 10−9	1.33
mrc1Δ swr1Δ	5 / 10	150 [125–200] x 10−9c	164 [136–218] x 10−9	0.92
mrc1-1-843	8 / 11	16.0 [12.0–31.3] x 10−9c	13.1 [9.82–25.6] x 10−9	1.22
mrc1-1-843 swr1Δ	3 / 11	87.3 [60.0–120] x 10−9c	175 [120–240] x 10−9d	0.50

^aObserved rate calculated by multiplying the GCR rates by the fraction of GCRs observed of a specific GCR type. 95% confidence intervals (CI) are displayed in square brackets.

^bPredicted rate calculated by multiplying the GCR rate for each strain by the fraction of GCRs observed in the wild-type strain. 95% confidence intervals are displayed in square brackets.

^cRate of a specific type of GCR in the mutant strain has a 95% CI that does not overlap with the 95% CI of the wild-type rate

^dPredicted rate based on the wild-type product distribution has a 95% CI that does not overlap with the 95% CI of the observed rate.