FIGURE 2.

Biofilm formation and bacterial growth in presence of nisin and ethanol in the culture broth of planktonic cells, (A) biofilm was quantified after 48 h incubation without nisin (solid color bars) or with 6 μg/mL nisin (diagonal striped bars) in the culture broth and with (0–8%) ethanol: dashed line (1 a.u.): above which it is considered positive biofilm formation, (B) bacterial growth was quantified after 48 h incubation without nisin (solid color bars) or with 6 μg/mL nisin (diagonal striped bars) in the culture broth and with (0–8%) ethanol; dashed line: initial bacterial population above which it is positive the bacterial growth of L. mesenteroides J32 (black bars) and O. oeni IS151 (gray bars).