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Addition of fresh culture broth containing binary combinations of nisin and ethanol on a preformed bacterial biofilm. The preformed biofilm of L. mesenteroides J32 in absence of antimicrobials, was quantified after 24 h incubation with fresh culture broth containing: 0 μg/ml nisin (black bars), 6 μ/mL nisin (diagonal striped bars), 200 μg/ml (horizontal striped bars) in binary combinations with ethanol (0–8%); dashed line: initial preformed biofilm.
