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Fluorescence microscopy images of L. lactis LM29 after 24 h incubation either in presence or in absence of ethanol in the culture broth. (A,B) Cells incubated in MRS broth. (C,D) Cells incubated in MRS broth with 12% ethanol. (A,C) Non-viable bacterial cells, with damaged cytoplasmic membrane, stained with propidium iodide that fluoresce red. (B,D) Viable cells, with intact cytoplasmic membrane, stained with Syto 9 that fluoresce green.
