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. 2018 Sep 11;8:13640. doi: 10.1038/s41598-018-31950-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Venn diagrams representing differentially nutrient (including absorption and concentration) GWAS hits was defined as the one had −log10(p) > 6.15 in high Mg²⁺ (A) and normal Mg²⁺ (B) of Arabidopsis. Each list in the venn diagram denotes by a transparent shape and overlaps shape indicates elements shared between lists or more often the corresponding counts. Four lists of input data of Fe-A, Fe-C, Mn-A and Mn-C were highlighted for both high Mg²⁺ and normal Mg²⁺ in green, blue, pink and yellow, respectively. Genome-wide association mapping results for the absorption and concentration of Fe and Mn in Arabidopsis under high Mg²⁺ (C) and normal Mg²⁺. (D) The ‘Manhattan setting’ plate for customized chromosome color, fixed pixel size, or dynamic pixel size proportional to p-values in Manhattan plot. The 5 chromosomes are represented by different shades of gray with the x-axis as the physical position. The highlighted positions represent the physical positions of the regions sharing common SNP between absorption and concentration of Fe and Mn.