Table 3.
Strains and plasmids used in this study.
| Strains | Characteristics | Source |
|---|---|---|
| C600 | F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 λ− | Ref.52 |
| Bth101 | F′ cya-99 araD139 galE15 galK16 rpsL1 (StrR) hsdR2 mcrA1 mcrB1 relA1 | Ref.60 |
| MR1-R | S. oneidensis, wild type strain, Rif R | Ref.54 |
| ΔtorE | MR1-R strain deleted of torE gene. | Ref.14 |
| ΔtorC | MR1-R strain deleted of torC gene. | This study |
| ΔtorEC | MR1-R strain deleted of torEC genes. | This study |
| ΔcymA | MR1-R strain deleted of cymA gene. | This study |
| Plasmids | Characteristics | Source |
| pBAD33 | Vector containing pBAD promoter with a p15A origin of replication | Ref.72 |
| pTorE | Coding sequence of the S. oneidensis TorE inserted in the pBAD33 vector | Ref.14 |
| pNapE | Coding sequence of the S. massilia NapE inserted in the pBAD33 vector | This study |
| pCydX | Coding sequence of the S. oneidensis CydX inserted in the pBAD33 vector | This study |
| pGFP-TorE | Coding sequence of the sfGFP fused to the S. oneidensis TorE with GSGGSG spanner and inserted in the pBAD33 vector | This study |
| pGFP-TorC | Coding sequence of the sfGFP fused to the S. oneidensis TorC and inserted in the pBAD33 vector | This study |
| pGFP-TorE; mCherry-TorC | Coding sequence of the sfGFP fused to the S. oneidensis TorE with GSGGSG spanner and and coding sequence of the mCherry fused to the S. oneidensis TorC with GSGGSG spanner, inserted in the pBAD33 vector | This study |
| pGFP-TorC; mCherry-TorE | Coding sequence of the sfGFP fused to the S. oneidensis TorC with GSGGSG spanner and and coding sequence of the mCherry fused to the S. oneidensis TorE with GSGGSG spanner, inserted in the pBAD33 vector | This study |
| pGFP-NapC | Coding sequence of the sfGFP fused to the S. massilia NapC with GSGGSG spanner and inserted in the pBAD33 vector | This study |
| pGFP-NapC; NapE | Coding sequence of the sfGFP fused to the S. massilia NapC with GSGGSG spanner and coding sequence of S. massilia NapE inserted in the pBAD33 vector | This study |
| pGFP-NapC; mCherry-NapE | Coding sequence of the sfGFP fused to the S. massilia NapC with GSGGSG spanner and coding sequence of mCherry fused to S. massilia NapE with GSGGSG spanner inserted in the pBAD33 vector | This study |
| pGFP-CymA | Coding sequence of the sfGFP fused to the S. oneidensis TorE and inserted in the pBAD33 vector | This study |
| pEC86 | CcmABCDEFGH genes from E. coli cloned in the pACYC184 vector | Ref.73 |
| pEB355 | pUT18C derivative, coding for the T18 domain of the adenylate cyclase of B. pertussis | Ref.60 |
| pT18 TorE | Sequence coding for S. oneidensis TorE cloned in frame at the 3 extremity of the sequence coding for the T18 domain into pEB355 | This study |
| pT18 TorC | Sequence coding for S. oneidensis TorC cloned in frame at the 3 extremity of the sequence coding for the T18 domain into pEB355 | This study |
| pT18 TorCm | Sequence coding for the 1- amino acids of S. oneidensis TorC cloned in frame at the 3 extremity of the sequence coding for the T18 domain into pEB355 | This study |
| pT18 MCP | Sequence coding for the SO_2117 MCP from S. oneidensis cloned in frame at the 3 extremity of the sequence coding for the T18 domain into pEB355 | This work |
| pT18 CydX | Sequence coding for S. oneidensis CydX cloned in frame at the 3 extremity of the sequence coding for the T18 domain into pEB355 | This work |
| pT18 napE | Sequence coding for S. massilia NapE cloned in frame at the 3 extremity of the sequence coding for the T18 domain into pEB355 | This work |
| pEB354 | pKT25 derivative, coding for the T25 domain of the adenylate cyclase of B. pertussis | Ref.60 |
| pT25 TorE | Sequence coding for S. oneidensis TorE cloned in frame at the 3 extremity of the sequence coding for the T25 domain into pEB354 | This work |
| pT25 torCm | Sequence coding for the 1- amino acids of S. oneidensis TorC cloned in frame at the 3 extremity of the sequence coding for the T25 domain into pEB354 | This work |
| pT25 NapE | Sequence coding for S. massilia NapE cloned in frame at the 3 extremity of the sequence coding for the T25 domain into pEB354 | This work |
| pT25 NapC | Sequence coding for S. massilia NapC cloned in frame at the 3 extremity of the sequence coding for the T25 domain into pEB354 | This work |