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. 2018 Sep 11;8:13638. doi: 10.1038/s41598-018-31835-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fatty dHepaRG cells activate a cell autonomous IL6 inflammatory response. (a) dHepaRG cells were treated with vehicle (Ctrl) or with sodium oleate 250 μM for 4 days. cDNAs were analyzed by qPCR with primers specific for the indicated genes and normalized to Actin. (b) Left panel: total protein extracts were analyzed by Immunoblotting with the indicated antibodies. Right panel: densitometric analysis (ImageJ software). (c) Cells were treated as in a) and supernatants were colllected to quantify secreted IL6 levels by ELISA assay. (d) Left panel: nuclear and cytoplasmic protein extracts from cells treated as in a) were analysed by Immunoblotting with the indicated antibodies. Right panel: densitometric analysis (ImageJ software). Histograms show fold induction of treated cells versus control; bars indicate S.D.; asterisks indicate p-value. Full-length blots are included in Supplementary Fig. 9.