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. 2018 Sep 11;8:13638. doi: 10.1038/s41598-018-31835-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of STAT3 in fatty dHepaRG. (a) Left panel: dHepaRG cells were treated with vehicle (Ctrl) or with sodium oleate 250 μM for 4 days, protein extracts were analyzed by immunoblotting with the indicated antibodies. Right panel: densitometric analysis (ImageJ software). (b) RNA transcripts were extracted from cells treated as in a) and cDNAs were analyzed with STAT3 specific primers and normalized to Actin. (c/d) dHepaRG cells were treated with vehicle (Ctrl) or with sodium oleate 250 μM for 4 days, and co-treated for the subsequent 18 hours with sodium oleate 250 μM and with Ruxolitinib 1 μM (c) or with sodium oleate 250 μM and NAC 10 mM (d). Left panels: protein extracts were analyzed by Immunoblot with the indicated antibodies. Right panels: densitometric analysis (ImageJ software). Histograms show relative protein level expressed as fold induction of treated cells versus control; bars indicate S.D.; asterisks indicate p-value. Full-length blots are included in Supplementary Fig. 10.