Skip to main content
. 2018 Sep 11;9(5):e01340-18. doi: 10.1128/mBio.01340-18

FIG 5 .

FIG 5 

The peptidoglycan biosynthetic pathway is sensitive to d-Ala levels. (A) P. aeruginosa PA14 dadA::Tn mutant cells grew better than the PA14 wild-type strain in the presence of a sub-MIC level of DCS. y-axis data represents absorbance (λ = 595 nm) after 16 h of growth. Adding d-Ala to the growth media enhanced the growth phenotype of the dadA::Tn mutant. (B) An increase in the concentration of exogenous d-Ala (in the growth media) reduced the GRABS score for dadA::Tn mutant cells compared to wild-type cells. (C) dadA::Tn mutant cells grown in LB media supplemented with d-Ala-d-Ala (15 mM) had a 30% decrease in the GRABS score compared to wild-type cells. (D) Transcription of ponA, dacC, murF, and ddl was reduced in P. aeruginosa PA14 dadA::Tn mutant cells compared to wild-type cells. These genes code for proteins that either release d-Ala into the periplasm (dacC and ponA) or utilize d-Ala as a substrate for peptidoglycan precursor synthesis (ddl and murF).