TABLE 1.

Relative fitness of strains carrying the tufB operon at different locations and orientations

Locationa	Relative fitnessb		Relative tufB expressionc
	wt	ΔtufA	wt	ΔtufA
246	0.98 ± 0.02	1.04 ± 0.03	1.06 ± 0.04	1.28 ± 0.02
276d	1.00 ± 0.03	1.00 ± 0.02	1.00 ± 0.04	1.00 ± 0.01
991	1.02 ± 0.03	0.90 ± 0.01	0.99 ± 0.03	0.91 ± 0.01
1555	0.95 ± 0.03	0.80 ± 0.01	0.93 ± 0.01	0.82 ± 0.02
2154	0.95 ± 0.01	0.73 ± 0.02	0.91 ± 0.03	0.71 ± 0.01

^aChromosomal location as the distance to OriC in kilobases.

^bRelative fitness ± standard deviation measured as the exponential growth rate. wt, wild type.

^cRelative tufB expression level ± standard deviation measured as transcriptional yfp fusion in a wild-type (wt) or ΔtufA strain. Values are relative to the values for their respective isogenic strains with the tufB operon in the original location.

^dNative tufB operon location.