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. 2018 Sep 11;8:13604. doi: 10.1038/s41598-018-31861-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NS5A and NS3 fluorescent foci are resolved as clusters of localisations by 3D-dSTORM. (a–e) NS5A, (f–j) NS3. (a,f) Wide-field fluorescence images of Huh7 cell 24 hours post-infection with JFH-1 and immunostained for NS5A or NS3. (b,g) 3D-dSTORM images of cell in (a,f). (c,h) Projections of 2 μm-thick volumes, 100 nm histogram bins. Regions of interest within (a and f) at higher magnification. (d,i) 3D-dSTORM images of regions shown in (c,h). Projections of 2 μm-thick volumes, 10 nm histogram bins. (e,j) Individual clusters of localisations (numbered in d,i). Projections of 1 μm-thick volumes, 5 nm histogram bins. Colour bar indicates localisation density per histogram bin. 3D-dSTORM images are sum projections over depth (z) smoothened with a Gaussian filter, σ = 20 nm.