Fig. 3.

Association of IL-37V1 with GCF Cytokines and DC IL-1β mRNA expression. a Z-Score Standardized Log GCF IL-1β Levels by IL-37 Variant 1 Haplotype. This figure reflects the Z-score for each of the six GCF mediators assayed in a sample of 107 subjects who were genotyped for the IL-37 V1 locus by pyrosequencing, assaying six mediators within the same GCF sample for each subject and normalizing to IL-37WT. There was an overall increase in the levels of these mediators expressed as a composite z score for each subject pooling each mediator and transforming using the 1.1 allele as zero (P = 0.026) and comparing 1.1 to 1.2 + 2.2. The increased expression of IL-1β and IL-8 were significant by pairwise comparisons comparing 1.1 to 2.2 at p = 0.046 and p = 0.03. b IL-1β secretion at various times by dendritic cells from IL-37WT-1.1 individuals and homozygous IL-37V1-2.2 after LPS stimulation. Levels of IL-1β mRNA (fold expression) are shown at LPS concentration stimulation using isolated peripheral blood monocytes differentiated to DCs from subjects who were pyrosequenced for IL-37WT-1.1 (group 1) or for IL-37V1-2.2 (group 2). The insert shows and overall significant enhanced level of IL-1β mRNA among group 2 (V1 2.2) over time expressed as area under curve (AUC) with standard error bars at p < 0.05