Fig. 7.

IL-37b expression in HEK293T cells transfected with IL-37bWT and Variants. a Representative picture of RFP positive HEK293T cells after transfection. Scale bar is 400 µm. b Western blot to detect IL-37b production in the cell lysates of HEK293T cells transient transfected with IL-37b WT and Variants plasmid. c IL-37b mRNA level in the cell lysates of HEK293T cells transient transfected with IL-37b WT and Variants plasmid. d IL-37b production in the supernatant and lysate of HEK293T cells co-transfected IL-37b (WT or V1) and caspase-1 plasmid. e The sketch of Transwell system to detect the suppressive activity of IL-37b WT and V1. f After LPS stimulation, IL-1β expression level in the supernatant of co-culturing THP-1 cells with HEK293T cells transfected by IL-37b WT or V1 plasmid. The results are expressed as the mean ± SD. **p < 0.01 (unpaired two-tailed Student’s t-test). Results are representative of three independent experiments