(A) Luminex analysis of serum from the different treatment groups at
one week after tumor cell introduction reveals a significantly lower presence of
systemic cytokines in the positive control mice (C+I/iPSC,
C+A/AC29) compared to PBS control mice (PBS/AC29). The C+I/AC29
group follows a similar trend as the positive control samples (C+I/iPSC
and C+A/AC29, ANOVA with Tukey’s multiple comparison test,
*p<0.05, **p<0.001,
***p<0.001). (B) Among C+I
vaccinated mice (C+I1 through C+I5/AC29), there was greater
unique vaccine-associated variance within the TILs, whereas PBS-vaccinated mice
(PBS1 through 5/AC29) demonstrated a higher uniformity among T-cells that are
commonly present in lymphoid organs (Figure S6C-D).