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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Cell Stem Cell. 2018 Feb 15;22(4):501–513.e7. doi: 10.1016/j.stem.2018.01.016

Figure 7. Adjuvant vaccination after tumor resection leads to clean RAs and reactivation of the immune system to target cancer cells.

Figure 7

(A) B16F0 tumor-bearing mice underwent R1 tumor resection, were randomized into different treatment groups, and were vaccinated with either C+I, CpG, or PBS for four weeks. (B) DNA from skin biopsies (*) in resection areas (RAs) showed a significant reduction in the percentage of tumor cells after four vaccination rounds with the C+I vaccine, as assessed by ddPCR. (C) Vaccination post-tumor resection led to a reduction of Th17 cells (CD4+CD62L+TCR-b+(IL-2/IL-17A); CD4+CD62L+CD44+TCR-b+(IL-17A)) and an increased presence of TNF-α expressing myeloid cells (CD11b+CD44+GR1hi(TNF-a)) and IL-4 expressing CD19+CD62L+CD44+ B-cells (n=8 PBS, n=10 CpG, n=10 C+I, mean±s.e.m., ANOVA with Tukey’s multiple comparison test, *p<0.05). SQ: subcutaneous injection.