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Influence of effectors on the DNA-binding activity of five TFs. A mixture of 0.5 pmol each of FITC-labeled rsd and rmf probes was incubated with increasing concentrations of TFs (lanes 1 to 6: 0, 0.5, 1, 2, 4, and 8 pmol, respectively) in the presence of 10 mM each of the following effectors: (A) AcP for ArcA, (B) AI-2 for McbR, (C) acetate for RcdA, (D) A-1 (normal HSL) for SdiA, and (E) ppGpp for SlyA.
