FIG 6.
Influence of the overexpression of five TFs on the expression of rsd and rmf genes. (A) The wild type was transformed with each of five TF expression plasmids. The wild-type and transformed cells were grown in LB medium. Cell growth was monitored by measuring the cell density. At 3 h after inoculation of overnight culture into fresh medium, 50 µM IPTG was added for induction of TF expression. RNA samples were taken at 1, 3, and 6 h after induction for measurement of mRNA for each TF. (B) The level of rsd mRNA was measured by using qRT-PCR at the three growth phases indicated by arrows in panel A. (C) The level of rmf mRNA was measured as in panel B. For both panels B and C, the level of mRNA is shown as the relative value to that at the 4-h culture of wild-type cells. The measurements were repeated three times, and each P value was calculated by using CT values of <0.05 for the wild-type and mutant strains.