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. 2018 Mar 24;99(3):527–535. doi: 10.1093/biolre/ioy072

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — The role of CCOC in follicle development. (A–C) Secondary follicle images stained with anti-GFP antibody (green), anti-GDF-9 antibody (red), and DAPI (blue). The arrows in (B) and (C) denote localization of GDF-9 in AcGFP1-positive CCOCs. (D–K) Time-lapse images of a cultured ovary in aOog1pro3.9 mouse. (D, F, H, J) Bright-field images. (E, G, I, K) AcGFP1 images. (D, E) 43 h culture. (F, G) 51 h culture. (H, I) 55 h culture. (J, K) 59 h culture. Notes: o, oocyte. g, granulosa cell. Scale bar, 100 μm.