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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Biochemistry. 2010 Apr 13;49(14):3024–3030. doi: 10.1021/bi902043u

Figure 2.

Figure 2.

Image of a nondenaturing 12% polyacrylamide gel to assess restriction digestion of three-way junctions. Lanes 1 and 8 contain a 5-base pair ladder with 15 and 50 base pair oligonucleotides marked with arrows. Lanes 2-4 correspond to the unmodified three-way junction without 2-aminopurine. Lanes 2 and 3 represent the products of digestion by DraI and BsaAI, respectively, and the mobilities of the faster bands are between the 15 and 20 base pairs. Lane 4 shows the three-way junction before digestion. Lanes 5-7 correspond to the modified three-way junction 3-CAG, and the mobilities of the faster bands are between the 15 and 20 base pairs. Lanes 5 and 6 represent the products of digestion by DraI and BsaAI, respectively. Lane 7 shows the three-way junction before digestion.