Table 2.
Melting Temperatures and Acrylamide Quenching Constants.
| Oligonucleotidea | Tm,abs (°C)b | Tm,fluor (°C)c | Kq (M−1)d |
|---|---|---|---|
| β-CAG | 75.9 (0.4) | 77.0 (0.6) | 7.5 (0.2) |
| α-CAG | 77.2 (0.1) | 78.7 (0.3) | 9.8 (0.5) |
| 1-CAG | 76.6 (0.1) | ND | 11.1 (0.2) |
| 3-CAG | 76.8 (0.8) | 77.0 (1.7) | 15.0 (0.2) |
| 4-CAG | 76.4 (0.1) | 77.1 (0.6) | 13.5 (0.7) |
| 5-CAG | 75.9 (0.3) | 77.6 (1.6) | 15.2 (0.7) |
| 6-CAG | 76.4 (0.6) | 76.5 (0.3) | 14.9 (0.4) |
| Unmodified | 77.2 (0.2) | ||
| SS-CAG | 9.6 (1.0) | ||
| DS-CAG | 8.2 (0.6) |
a
Labels pertain to structure in Fig. 1A.
b
Melting temperatures derived from hyperchromic absorbance changes at 260 nm. Standard deviations provided in parentheses.
c
Melting temperatures derived from fluorescence intensity changes of substituted 2- aminopurines. The value for 1-AP was not determined (see Fig. 3). Standard deviations provided in parentheses.
d
Acrylamide quenching constants.