Fig. 3.

Western blot analyses of basal and insulin-stimulated phosphorylation of Akt in the muscle. Phosphorylation levels of Akt (Ser⁴⁷³ and Thr³⁰⁸) and total Akt were analyzed from protein extracts from the gastrocnemius of WT (green bar), ArKO (red bar), and ArKO/E2 (orange bar) mice fed, fasting for 19 h, and at 10 or 60 min after intraperitoneal injection of insulin into fasted mice. (A) Representative results of western blot analysis are shown. (B) Phosphorylated levels of Akt were normalized to total Akt (n = 6 for each group). *p < 0.05; compared among WT, ArKO, and ArKO/E2 mice. Unaltered blots are shown in Supplementary Fig. 2.