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. 2018 Sep 11;4(9):e00772. doi: 10.1016/j.heliyon.2018.e00772

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© 2018 The Authors. Published by Elsevier Ltd.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — RT-qPCR analysis of genes related to gluconeogenesis and lipogenesis in the liver. Quantitative RT-PCR analysis of mRNA of genes related to gluconeogenesis (Pklr, Pck1, and G6pc) and lipogenesis (Me1, Fasn, and Scd1) were performed on cDNA derived from hepatic total RNA of WT (green bar), ArKO (red bar), and ArKO/E2 (orange bar) mice. Livers were collected from fed (F), fasted for 19 h (f), and at 60 min after insulin-injected mice (I). Expression data from 7 samples per experimental group are shown as the mean ± SD following normalization for ribosomal protein L19 mRNA expression. * indicates significant difference.