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RT-qPCR analysis of genes downregulated by insulin. Livers were collected from fed (F), fasted (f), and at 60 min after insulin-injected fasted (I) WT (green bar), ArKO (red bar), and E2-supplemented ArKO (orange bar) mice. Expression data from 7 samples per experimental group are shown as the mean ± SD following normalization to ribosomal protein L19 mRNA expression. * indicates significant difference.
